Staff Scientist
ORNL
Research
My research interests lie in understanding and applying laser-based mass spectrometric techniques for biomolecule analysis. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a widely-used technique for analysis of proteins, peptides, and DNA. Using two commercial MALDI-MS instruments and robotic sample preparation capabilities, we are currently refining methods for identifying and characterizing proteins using proteolytic digestion combined with mass spectrometry. To study protein-protein complexes and interactions, we are exploring the powerful combination of crosslinking with subsequent mass spectrometric analysis. As an alternative to gel- or hybridization-based detection of PCR products, we have explored the possibility of using MALDI combined with primers especially designed to yield lower-molecular-weight PCR products.
Education
- PhD: Chemistry, University of Wisconsin, Madison (1990)
- BS: Chemistry, University of Tennessee, Knoxville (1984)
Publications
Yang, X.; Tschaplinski, T.J.; Hurst, G.B.; Jawdy, S.; Abraham, P.E.; Lankford, P.K.; Adams, R.M.; Shah, M.B.; Hettich, R.L.; Kalluri, U.C.; Gunter, L.E.; Pennacchio, C.; Tuskan, G.A. “Discovery and annotation of small proteins using genomics, proteomics and computational approaches.” Genome Research 2011, Published in Advance March 2. doi:10.1101/gr.109280.110.
Giannone, R.J.; McDonald, W.H.; Hurst, G.B.; Shen, R.-F.; Wang, Y.; Liu, Y. “The protein network surrounding the human telomere repeat binding factors TRF1, TRF2, and POT1.” PLOS ONE 2010, 5, e12409. doi:10.1371/journal.pone.0012407.
Kalluri, U.C., Hurst, G.B., Lankford, P.K., Ranjan, P., and Pelletier, D.A. ”Shotgun proteome profile of Populus developing xylem.” Proteomics 2009, 9, 4871-4880. DOI 10.1002/pmic.200800854.
Yang, S., Pappas, K.M., Hauser, L.J., Land, M.L., Chen, G.L., Hurst, G.B., Pan, C., Kouvelis, V.N., Typas, M.A., Pelletier, D.A., Klingeman, D.M., Chang, Y.J., Samatova, N.F.,Brown, S.D.“Improved genome annotation for Zymomonas mobilis.” Nature Biotechnology2009, 27, 893-894.
Edwards, A.N., Fowlkes, J.D., Owens, E.T., Standaert, R.F., Pelletier, D.A., Hurst, G.B., Doktycz, M.J., Morrell-Falvey, J.L. “An in vivo imaging-based assay for detecting protein interactions over a wide range of binding affinities.” Anal. Biochem. 2009, 395,166-177.
Hervey, W.J. IV, Khalsa-Moyers, G., Lankford, P.K., Owens, E.T., McKeown, C.K., Lu, T.-Y., Foote, L.J., Asano, K.G., Morrell-Falvey, J.L., McDonald, W.H., Pelletier, D.A., Hurst, G.B., “Evaluation of Affinity-Tagged Protein Expression Strategies using Local and Global Isotope Ratio Measurements.” J. Proteome Research 2009, 8, 3675-3688. DOI 10.1021/pr801088f.
Raman, B., Pan, C., Hurst, G.B., Rodriguez, M. Jr., McKeown, C.K., Lankford, P.K., Samatova, N.F., Mielenz, J.R. “Impact of Pretreated Switchgrass and Biomass Carbohydrates on Clostridium thermocellum ATCC 27405 Cellulosome Composition: A Quantitative Proteomic Analysis.” PloS One 2009, 4(4): e5271. doi: 10.1371/journal.pone.0005271.
Taylor, R.C., Singhal, M, Daly, D.S., Gilmore, J., Domico, K., White, A.M., Auberry, D.L., Auberry, K.J., Hooker, B., Hurst, G., McDermott, J., McDonald, W.H., Pelletier, D., Schmoyer, D., Cannon, W.R. “An analysis pipeline for the inference of protein-protein interaction networks,” Int. J. Data Mining and Bioinformatics (accepted, 2009).
Sharp, J.L., Borkowski, J.J., Schmoyer, D.D., Daly, D.S., Purvine, S., Cannon, W.R., Hurst, G.B., “Statistically Appraising Process Quality of Affinity-Isolation Experiments,” Comp. Stat. Data Analysis 2009, 53, 1720-1726.