Biology and Biomedical Sciences Group Leader
ORNL
Research
Neutron scattering studies are greatly enhanced by the design and production of specific, random and uniform hydrogen/deuterium labeled biological macromolecules that permit selected parts of macromolecular structures to be highlighted and analyzed in situ. CSMB has established a Bio-Deuteration Laboratory for in vivo production of hydrogen/deuterium-labeled bio-macromolecules for research at HFIR and SNS. The Bio-Deuteration Laboratory provides neutron users with access to facilities and expertise for cloning, protein expression, purification and characterization of deuterium-labeled biological macromolecules and trains visiting researchers in the use and application of these techniques. These capabilities will allow our facility users to design and produce proteins, complexes and macromolecular assemblies that are optimized for neutron scattering analysis and will enable these data to be used to construct biologically meaningful models of protein complexes, assemblies and hierarchical structures.
Publications
Quantum Model of Catalysis Based on a Mobile Proton Revealed by Subatomic X-ray and Neutron Diffraction Studies of h-aldose Reductase, Myles, D.A.A., M.P. Blakeley, F. Ruiz, R. Cachau, I. Hazemann, F. Meilleur, A. Mitschler, S. Ginell, P. Afonine, O. Ventura, A. Cousido-Siah, M. Haertlein, A. Joachimiak, and A. Podjarny, PNAS, 6, 1844-1848 (2008).
The Bio-SANS Small-angle Neutron Scattering Instrument at Oak Ridge National Laboratory, Heller, W.T., G.W. Lynn, V.S. Urban, K.L. Weiss, and Myles, D.A.A., Neutron News (2008).
The Center for Structural Molecular Biology (CSMB) at Oak Ridge National Laboratory (ORNL) Heller, W.T., G.L. Lynn, A.N. Raghavan, V.S. Urban, K.L. Weiss, Y. Mo, and Myles, D.A.A., The 2007 Meeting of the American Crystallographic Association, Salt Lake City, Utah (2007).
The Center for Structural Molecular Biology (CSMB) at Oak Ridge National Laboratory (ORNL) Heller, W.T., G.W. Lynn, A.N. Raghavan, V.S. Urban, G.D. Wignall, K.L. Weiss, and Myles, D.A.A., American Conference on Neutron Scattering (2006).
The Center for Structural Molecular Biology (CSMB) at Oak Ridge National Laboratory Heller, W.T., G.W. Lynn, A.N. Raghavan, V.S. Urban, G.D. Wignall, K.L. Weiss, and Myles, D.A.A., American Conference on Neutron Scattering, St. Charles, Illinois (2006).
Neutron Laue Macormolecular Crystallography Myles, D.A.A., F. Meilleur, and M.P. Blakeley, European Biophysics Journal, 35, 611-620 (2006).
Neutron Protein Crystallography: Current Status and a Brighter Future Myles, D.A.A., Current Opinion in Structural Biology, 16, 1-8 (2006).
Preliminary Neutron Diffraction Studies of Escherichia Coli Dihydrofolate Reductase Bound to the Anticancer Drug Methotrexate Myles, D.A.A., B.C. Bennett, F. Meilleur, E.E. Howell, and C.G. Dealwis, Acta Crystallographica, D61, 574-579 (2005).
Production and X-ray Crystallographic Analysis of Fully Deuterated Cytochrome P450cam Myles, D.A.A., F. Meilleur, M.T. Dauvergne, and I. Schlichting, Acta Crystallographica, D61, 539-544 (2005).
High-resolution Neutron Protein Crystallography with Radically Small Crystal Volumes: Application of Perdeuteration to Human Aldose Reductase Myles, D.A.A., I. Hazemann, M.T. Dauvergne, M.P. Blakeley, F. Meilleur, M. Hartlein, A. Van Dorsselaer, and A. Podjarny, Acta Crystallographica, D61, 1413-1417 (2005).
Neutron Diffraction Investigations of L- and D-alanine at Different Temperatures: The Search for Structural Evidence for Parity Violation Myles, D.A.A., C.C. Wilson, M. Ghosh, L.N. Johnson, and W. Wang, New Journal of Chemistry, 29, 1318-1322 (2005).