Professor, Director of Genome Science & Technology
A plant can be regarded as a processor that senses environmental light conditions and transduces this information to shape development of the organism to achieve optimal growth (photomorphogenesis). Our goal is to understand the mechanistic basis for these fascinating events. This research employs cell biology and molecular genetics and is greatly facilitated by the tools of genomics, which have become easily accessible in our model organism, Arabidopsis thaliana. These include the completed Arabidopsis genome sequence, T-DNA knockout insertion lines, transposon mutagenesis, facile generation of transgenic plants, and more. Part of our effort is to contribute to the tools of proteomics and functional genomics, specifically, by optimizing a real-time in vivo method to study protein-protein interactions, termed Bioluminescence Resonance Energy Transfer (BRET). Another major project investigates how light signals modulate the subcellular and subnuclear localization of key regulatory proteins in Arabidopsis thaliana. These proteins are highly conserved among higher eukaryotes and can be studied well in Arabidopsis given the unique combination of biochemistry, genetics, and cell biology possible in this organism. We are particularly intrigued by a novel biochemical line of communication between the cellular machineries for translation (protein synthesis) and proteolysis (protein degradation). Interestingly, derailment of this process may underlie certain cases of cancer in humans.
- Postdoctoral Fellow: Yale University, New Haven, CT (1992-1996)
- PhD: University of East Anglia and John Innes Institute, Norwich, United Kingdom (1992)
- MS: University of Freiburg, Germany (1988)
Missra, A., Ernest, B., Lohoff, T., Jia, Q., Satterlee, J., Ke, K., and von Arnim, A.G. 2015. The circadian clock modulates global daily cycles of mRNA ribosome loading. Plant Cell 27: 2582-2599.
Zhou, F., Roy, B., Dunlap, J.R., Enganti, R., and von Arnim, A.G. 2014. Translational control of Arabidopsis meristem stability and organogenesis by the eukaryotic translation factor eIF3h. PLoS One 9:e95396.
von Arnim, A.G., Jia, Q., Vaughn, J.N. (2014). Review: Regulation of plant translation by upstream open reading frames. Plant Science 214:1-12.
Missra, A and von Arnim, A.G. (2014). Analysis of mRNA translation states over the diurnal cycle by polysome microarray. In: Plant Circadian Networks (Dorothee Staiger, Ed.). Methods in Molecular Biology 1158:157-174.
Tiruneh, B.S., Kim, B.H., Gallie, D.R., Roy, B., and von Arnim, A.G. (2013). The global translation profile in a ribosomal protein mutant resembles that of an eIF3 mutant. BMC Biology 11:123.
Roy, B., and von Arnim, A.G. (2013). Translational regulation of cytoplasmic mRNAs. In: The Arabidopsis Book. K Torii and C Chang eds. e0165.
Kim, B.H., Malec, P., Waloszek, A., von Arnim, A.G. (2012). Arabidopsis BPG2: A phytochrome regulated gene whose protein product binds to plastid ribosomal RNAs. Planta 236: 677-690.
Vaughn, J.N., Ellingson, S.R., Mignone, F., and von Arnim, A.G. (2012). Known and Novel Post-Transcriptional Regulatory Sequences are Conserved across Plant Families. RNA 18: 368-384. January 11 2012. 10.1261/rna.031179.111
Vaughn, J.N. and von Arnim, A.G. (2012). uORF-Mediated Translational Control in Eukaryotes. In: W. Dubitzky, O. Wolkenhauer, K. Cho & H. Yokota (eds.), Encyclopedia of Systems Biology, Springer Science & Business Media, LLC. DOI 10.1007/978-1-4419-9863-7 (in press).
Kim, B.H. and von Arnim, A.G. (2011). Molecular approaches to the study of plant development. In: Plant Tissue Culture, Development, and Biotechnology, Robert N. Trigiano and Dennis J. Gray, eds. CRC Press, Boca Raton, FL.
Roy, B., Copenhaver, G.P., and von Arnim, A.G. (2011). Fluorescence-tagged transgenic lines reveal genetic defects in pollen growth - application to the eIF3 complex. PLoS One 6:e17640.
Zhou, F., Roy, B., and von Arnim, A.G. (2010). The translation initiation factor eIF3h cooperates with the large ribosomal subunit in mediating translation reinitiation on mRNAs harboring upstream open reading frames. BMC Plant Biology 10:193
Roy, B., Vaughn, J.N., Kim, B.H., Zhou, F., Gilchrist, M.A., and von Arnim, A.G. (2010) The h subunit of eIF3 promotes reinitiation competence during translation of mRNAs harboring upstream open reading frames. RNA. 16:748-61.
Li, J.F., Park, E., von Arnim A.G., and Nebenführ, A. (2009) The FAST technique: a simplified Agrobacterium-based transformation method for transient gene expression analysis in seedlings of Arabidopsis and other plant species. PlantMethods. 5:6.
Kim, B.H. and von Arnim, A.G.. (2009) FIERY1 regulates light-mediated repression of cell elongation and flowering time via its 3'(2'),5'-bisphosphate nucleotidase activity. Plant J. 58:208-19.
Stahle, M.I., Kuehlich, J., Staron, L., von Arnim, A.G., and Golz JF. (2009) YABBYs and the transcriptional corepressors LEUNIG and LEUNIG_HOMOLOG maintain leaf polarity and meristem activity in Arabidopsis. Plant Cell. 21:3105-18.
Li, J.-F., Park, E., von Arnim, A.G. and Nebenführ, A. (2009). Fast Agrobacterium-mediated seedling transformation in Arabidopsis and other plant species. Plant Methods 5:6.
Woo, J.C. and von Arnim, A.G. (2008) Mutational optimization of the coelenterazine-dependent luciferase from Renilla. Plant Methods. 4:23.
Kim, B.H., and von Arnim, A.G. (2008). FIERY1 regulates light-mediated repression of cell elongation and flowering time via its 3’(2’),5’-bisphosphate nucleotidase activity. Plant Journal 58:208-19.
Woo, JC, and von Arnim, AG (2008) Mutational optimization of the coelenterazine-dependent luciferase from Renilla. Plant Methods (in press).
Woo, JC, Howell, MH, and von Arnim, AG (2008) Structure function studies on the active site of the coelenterazine-dependent luciferase from Renilla.
Protein Science 17: 725-735.
Yahalom, A, Kim, TH, Roy, R, Zinger, R, von Arnim, AG, and Chamovitz, DA (2008) Arabidopsis eIF3e is regulated by the COP9 signalosome and impacts development and protein translation. Plant Journal 53: 300-311.