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Judson Hervey BS - Biology, West Liberty State College Oak Ridge National Laboratory |
Description of Research
Mentor(s): Greg Hurst & Dale Pelletier
Under the co-mentorship of Drs. Hurst and Pelletier, my research involves the identification and characterization of microbial protein complexes in the Genomes to Life Center for Cellular and Molecular Systems. Within our protein complex analysis pipeline, we currently use affinity-based methods to first isolate protein complexes followed by liquid chromatography tandem mass spectrometry (LC-MS/MS) methods to identify and characterize the constituent components of the protein complexes.
Currently, I am working on methods to distinguish between specific and non-specific protein interactions among the affinity-isolated protein complexes using isotopic differentiation. Methods of affinity-isolation are often laden with non-specific, background interactions formed during the isolation process, not during protein complex formation or function in vivo. Distinguishing such artifactual interactions over biologically-relevant interactions will contribute to an enhanced understanding of microbial cellular function.
Rotation Summary
Mentor: Steve Wilhelm - Fall 2002
Proteomic Analysis of Synechocystis PC6803 under Iron Stress.
The sequenced cyanobacterium, Synechocystis was grown under differing concentrations of iron and subjected to liquid chromatography Mass Spectrometry-Mass Spectrometry (LC-MS/MS). A differential protein expression pattern was generated between colonies under iron stress and those under normal conditions.
Mentor: Gary J. Van Berkel - Spring 2003
Experimental Simulations in the Identification of Biological Threat Agents.
The current geopolitical climate necessitates the detection and identification of potentially threatening agents, such as toxins, bacteria, and viruses. Although different approaches exist to perform this critical action, each has inherant technology-specific flaws. In this rotation, the method of "shotgun" proteomics was evaluated by performing two series of experimental simulations. In the battlefield scenario, a complex mixture of proteins was analyzed on a time-dependant basis. Alternatively, in the homeland security scenario, time-dependance was negated for more accurate detection.
Mentor: Loren Hauser - Summer 2003
Chemotaxis Operon Structure of Rhodopseudomonas palustris.
Abstract
Mentor: Yie Liu - Summer 2003
Affinity Purification of Telomere Repeat Factor 2 (TRF2) and Cell Division Cycle Protein 14 B (CDC14B).
Abstract
Publications
- Nathan C. Verberkmoes, W. Judson Hervey, Manesh Shah, M Land, Loren J. Hauser, Frank W. Larimer, Gary J Van Berkel, Douglas E. Goeringer. 2005. Evaluation of "Shotgun" Proteomics for Identification of Biological Threat Agents in Complex Environmental Matrixes: Experimental Simulations. Analytical Chemistry. 77(3): 923-932. PubMed
- Strader MB, Tabb DL, Hervey WJ IV, Pan C, Hurst GB. 2006. Efficient and Specific Trypsin Digestion of Microgram to Nanogram Quantities of Proteins in Organic-Aqueous Solvent Systems. Analytical Chemistry 2006 Jan 1;78(1):125-134
Abstracts of Posters and Talks
- W. Judson Hervey, Jane Razumovskaya, Nathan C. VerBerkmoes, Miriam Land, Loren J. Hauser, Frank W. Larimer, Ying Xu, Gary J. VanBerkel, and Douglas E. Goeringer. 2003. Computational and Experimental Simulations for Mass Spectrometry-based Identification of Biological Weapons. Poster, 51st American Society for Mass Spectrometry (ASMS) Conference - Montreal, Quebec.
- Hervey WJ IV, VerBerkmoes NC, Shah M, Land M, Hauser LJ, Pelletier DA, Larimer FW, Hurst GB. 2005. Proteomic Verification of Signal Peptide Prediction Algorithms in Rhodopseudomonas palustris. Poster, 53rd American Society for Mass Spectrometry (ASMS) Conference - San Antonio, Texas
- Strader MB, Tabb DL, Hervey WJ IV, Pan C, Hurst GB. 2005. Organic Solvents Improve Trypsin Digestion Efficiency and Specificity for Digesting Limited Protein Sample Amounts. Poster, 53rd American Society for Mass Spectrometry (ASMS) Conference - San Antonio, Texas