Greg Hurst

Keywords:

Mass spectrometry, analytical chemistry, MALDI, proteins, DNA

Research Area:

Description of Research:

My research interests lie in understanding and applying laser-based mass spectrometric techniques for biomolecule analysis. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) is a widely-used technique for analysis of proteins, peptides, and DNA. Using two commercial MALDI-MS instruments and robotic sample preparation capabilities, we are currently refining methods for identifying and characterizing proteins using proteolytic digestion combined with mass spectrometry. To study protein-protein complexes and interactions, we are exploring the powerful combination of crosslinking with subsequent mass spectrometric analysis. As an alternative to gel- or hybridization-based detection of PCR products, we have explored the possibility of using MALDI combined with primers especially designed to yield lower-molecular-weight PCR products.

Contact Information

Greg Hurst
Analytical Technologies for Bioenergy and the Envrionment
Staff Scientist, Oak Ridge National Laboratory

ORNL
PO Box 2008 MS6131
Oak Ridge, TN 37831-6131

Email: hurstgb@ornl.gov

Degrees

PhD: Chemistry, University of Wisconsin, Madison (1990)

BS: Chemistry, University of Tennessee, Knoxville (1984)